Mechs vs. Minions turning on a rune (mission 3)

Hello, I was curious if doing a turn on a rune space during mission 3 would disable the rune shield. I ask because I had a Cyclotron card that damaged the boss while I was on a yellow rune, then my turn ended with a fuel tank which had me turn on that yellow rune and I wasn't sure if that would re-disable the shield or not. We ended up counting it as a disable but we barely won, so I feel like I cheezed it... any clarification on this would be appreciated.
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